Plipastatins: new inhibitors of phospholipase A2, produced by Bacillus cereus BMG302-fF67. I. Taxonomy, production, isolation and preliminary characterization.
نویسندگان
چکیده
Plipastatins have been isolated as part of a program designed to find inhibitors of porcine pancreatic phospholipase A2. They were purified from fermentation broth of Bacillus cereus BMG302-fF67 and finally separated into four fractions by reverse phase HPLC. The respective fractions were designated as plipastatins A1, A2, B1 and B2. Plipastatins also inhibited phospholipases C and D.
منابع مشابه
Plipastatins: new inhibitors of phospholipase A2, produced by Bacillus cereus BMG302-fF67. III. Structural elucidation of plipastatins.
Plipastatins are new inhibitors of phospholipase A2 produced by Bacillus cereus BMG302-fF67. Structures of the plipastatins have been determined by UV, mass and NMR spectrometries and chemical degradation. The carboxyl group of the C-terminal L-isoleucine of plipastatinic acid has been shown to form a lactone linkage with the hydroxyl group of L-tyrosine. The total structure of plipastatins has...
متن کاملPlipastatins: new inhibitors of phospholipase A2, produced by Bacillus cereus BMG302-fF67. II. Structure of fatty acid residue and amino acid sequence.
Plipastatins, new inhibitors of phospholipase A2, were produced by a strain classified as Bacillus cereus BMG302-fF67. The plipastatins are a family of acylated decapeptides which differ from each other by amino acid composition and the nature of the fatty acid side chain. The fatty acids have been shown to be 3(R)-hydroxyhexadecanoic acid (n-C16h3) and 14(S)-methyl-3(R)-hydroxyhexadecanoic aci...
متن کاملCharacterization of antimicrobial metabolites produced by halophilic Actinomyces isolated from Aran-Bidgol and Maharlu lakes
Introduction: The purpose of this study was isolation of halophilic Actinomyces and the evaluation of their potential for producing antimicrobial metabolites. Material and method: To isolate antimicrobial metabolites producing Actinomyces, culture supernatant of 51 halophilic Actinnomyces isolates were assessed against Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus, Staphylococcu...
متن کاملIsolation of Halophilic Bacteria from Maharlu salt Lake - Iran and their evaluation for the production of bioactive compounds
Halophilic bacteria grow over a wide range of salt concentrations. In this study we aimed to isolate and screen out the halophilic bacteria and to determine their activity for production of the bioactive compounds. A total of 50 water, sediments and soil samples were collected from Maharlu salt lake in southern region of Fars-Iran and subjected for isolation of the bioactive compound producing ...
متن کاملNMR structure of antibiotics plipastatins A and B from Bacillus subtilis inhibitors of phospholipase A(2).
Plipastatins A and B are antifungal antibiotics belonging to a family of lipopeptides capable of inhibiting phospholipase A(2) (PLA(2)) and are biosynthesised under certain circumstances by Bacillus subtilis. U-(15)N plipastatins A and B were obtained from cultures of the strain NCIB 8872 on a Landy medium modified for stable-isotope labelling by the substitution of the L-glutamic acid used as ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The Journal of antibiotics
دوره 39 6 شماره
صفحات -
تاریخ انتشار 1986